Flow Cytometry Core

Core Summary:  A Harvard University affiliated core facility supported by the Center for AIDS Research (CFAR). The Cytometry core provides Equipment consultation, and data analysis overseen by dedicated personnel. The same services are also available to outside researchers. Our expertise includes Non-Human Primate (NHP) research, and infectious diseases.

The Core team is committed to continuously expand with the latest software and lasers to better assist peer researchers.


 

Personnel

Director:  Keith Reeves, PhD
Flow Core Lab Manager:    Michelle Lifton                                
                                               Phone: (617) 735-4512
                                               Email:  mlifton@bidmc.harvard.edu  
Flow Core Lab Assistants:  Mary Laughridge
                                               Phone:  (617) 735-4515
                                               Email:  mlaughri@bidmc.harvard.edu
                                               Vaishnavi Parthasarathy
                                               Phone:  (617) 735-4505
                                               Email:  vparthas@bidmc.harvard.edu

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Services

  •          Data analysis and interpretation
  •          Experiment consultation and set-up
  •          Fluorescence activated cell sorting
  •          Panel development
  •          Antibody conjugation
  •          Assays (special development)

                                                                    Equipment

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BD FACSAria II

COMS approved facility allows for sterile sorting of fixed and unfixed human, non-human primate, and other animal specimens for HCV and HIV/AIDS research.  BD FACS Aria II cell sorter equipped within a Bioprotect IV LE biosafety cabinet operates with 4 solid state lasers: 405nm violet measuring up to 8 parameters, 488nm blue measuring up to 2 parameters in addition to FSC and SSC, 532nm green measuring up to 5 parameters, and a 628nm red measuring up to 3 parameters, allowing sorting panels of up to 18 fluorescent markers.

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BD LSRII

Two BD LSR II analyzers, each equipped with 4 solid state lasers: 405nm violet measuring up to 7 parameters,  488nm blue measuring up to 5 parameters in addition to FSC and SSC,  a 640nm red measuring up to 3 parameters, and a 355 nm UV laser measuring3 parameters allowing panels of up to 18 fluorescent markers

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BD LSRFortessa X14

Special research product with a choice of 3 solid state lasers a 405nm violet measuring up to 6 parameters,  488nm blue measuring up to 2 parameters in addition to FSC and SSC,  a 561nm Yellow/Green measuring up to 4 parameters allowing panels of up to 12 fluorescent markers

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Siemens ADVIA 120 Hematology System

Mmultispecies analyzing system for complete blood count and differential counts