Alan N. Engelman, Ph.D.

Education

• Tufts University, Medford, MA, B.S.Ch.E., 1981 (Chemical Engineering)
• Tufts University, Medford, MA, M.S., 1985 (Life Science Engineering)
• Tufts University School of Medicine, Boston, MA, Ph.D., 1990 (Molecular Biology)

Training

• NIDDK/National Institutes of Health, Bethesda, MD, 1995 (HIV-1 Integration)

Appointments

• 1995-2001, Assistant Professor, Department of Pathology, Harvard Medical School, Boston, MA
• 2001-2009, Associate Professor, Department of Pathology, Harvard Medical School, Boston, MA
• 2009-2012, Associate Professor, Department of Medicine, Harvard Medical School, Boston, MA
• 2012-present, Professor, Department of Medicine, Harvard Medical School, Boston, MA
• 2016-2018, Affiliated Faculty, Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA
• 2018-present, Affiliated Faculty, Department of Microbiology, Harvard Medical School, Boston, MA

Hospital Appointments

• 1995-1996, Assistant Professor of Pathology, Division of Human Retrovirology, Dana-Farber Cancer Institute, Boston, MA
• 1997-2001, Assistant Professor of Pathology, Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, MA
• 2001-2009, Associate Professor of Pathology, Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, MA
• 2009-2012, Associate Professor of Medicine, Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, MA
• 2009-2012, Associate Professor of Medicine, Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Boston, MA
• 2012-2015, Professor of Medicine, Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, MA
• 2012, Professor of Medicine, Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Boston, MA
• 2013-present, Professor of Medicine, Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA
• 2015-present, Professor of Medicine, Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA

Honors

• 1995, R-AXIS Golden Crystal Award, 2nd place, Molecular Structure Corporation, The Woodlands, TX
• 2010, MERIT Award, NIAID/NIH
• 2012, Master of Arts, Honoris Causa, Harvard University
• 2014, Keynote Speaker, 5th International Conference on Retroviral Integration, Asilomar, California
• 2014, Elected Fellow, American Association for the Advancement of Science
• 2016, Elected Fellow, American Academy of Microbiology

Publications

• Sadri G, Nadakal ST, Lauer W, Kos J, Singh PK, Elliott E, Kaiser CW, Ford EE, Richardson N, Shields KM, Hudson E, Linden NL, Danesh A, Powell J, Warburton P, Soto J, Emery M, Deikus G, Lee GQ, Lamers SL, Reynolds SJ, Galiwango R, Prodger JL, Tomusange S, Kityamuweesi T, Han T, Jones RB, Tobian AAR, Engelman AN, Sebra R, Morgello S, Redd AD, Sachs D, Rouchka E, Smith ML. Development and validation of HIV SMRTcap for the characterization of HIV-1 reservoirs across tissues and subtypes. PLoS Pathog. 2026 Jan 13;22(1):e1013171. doi: 10.1371/journal.ppat.1013171. PMID: 41529060; PMCID: PMC12851485. [Article]


• Hou Z, Shen Y, Fronik S, Shen J, Shi J, Xu J, Chen L, Hardenbrook N, Engelman AN, Aiken C, Zhang P. HIV-1 nuclear import is selective and depends on both capsid elasticity and nuclear pore adaptability. Nature Microbiology. 2025 Aug;10(8):1868-1885. doi: 10.1038/s41564-025-02054-z. Epub 2025 Jul 7. [Article]


• Engelman AN, Grandgenett DP, Maertens GN, Yoder KE, Kvaratskhelia M. Retrointegration2023-Papers from the 7th International Conference on Retroviral Integration. Viruses. 2025 Jun 23;17(7):879. doi: 10.3390/v17070879. [Article]


• Lagadec F, Singh PK, Calmels C, Lapaillerie D, Lindemann D, Parissi V, Cherepanov P, Engelman AN, Lesbats P. Timed chromatin invasion during mitosis governs prototype foamy virus integration site selection and infectivity. Nucleic Acids Res. 2025 May 22;53(10):gkaf449. doi: 10.1093/nar/gkaf449. [Article]


• Briganti, L., Annamalai, A. S., Bester, S. M., Wei, G., Andino-Moncada, J. R., Singh, S. P., Kleinpeter, A. B., Tripathi, M., Nguyen, B., Radhakrishnan, R., Singh, P. K., Greenwood, J., Schope, L. I., Haney, R., Huang, S.-W., Freed, E. O., Engelman, A. N., Francis, A. C., & Kvaratskhelia, M. (2025). Structural and mechanistic bases for resistance of the M66I capsid variant to lenacapavir. mBio, 16(5), e0361324. https://doi.org/10.1128/mbio.03613-24 [Article]


• Chaudhuri, E., Jang, S., Chakraborty, R., Radhakrishnan, R., Arnarson, B., Prakash, P., Cornish, D., Rohlfes, N., Singh, P. K., Shi, J., Aiken, C., Campbell, E., Hultquist, J., Balsubramaniam, M., Engelman, A. N., & Dash, C. (2025). CPSF6 promotes HIV-1 preintegration complex function. Journal of virology, 99(5), e0049025. https://doi.org/10.1128/jvi.00490-25 [Article]


• Ingram, Z., Kline, C., Hughson, A. K., Singh, P. K., Fischer, H. L., Radhakrishnan, R., Sowd, G. A., Dos Santos, N. F. B., Ganser-Pornillos, B. K., Watkins, S. C., Kane, M., Engelman, A. N., & Ambrose, Z. (2025). Spatiotemporal binding of cyclophilin A and CPSF6 to capsid regulates HIV-1 nuclear entry and integration. mBio, 16(4), e0016925. https://doi.org/10.1128/mbio.00169-25 [Article]


• Huang, S. W., Briganti, L., Annamalai, A. S., Greenwood, J., Shkriabai, N., Haney, R., Armstrong, M. L., Wempe, M. F., Singh, S. P., Francis, A. C., Engelman, A. N., & Kvaratskhelia, M. (2025). The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir. PLoS pathogens, 21(1), e1012862. https://doi.org/10.1371/journal.ppat.1012862 [Article]


• Rohlfes, N., Radhakrishnan, R., Singh, P. K., Bedwell, G. J., Engelman, A. N., Dharan, A., & Campbell, E. M. (2025). The nuclear localization signal of CPSF6 governs post-nuclear import steps of HIV-1 infection. PLoS pathogens, 21(1), e1012354. https://doi.org/10.1371/journal.ppat.1012354 [Article]


• Sooin Jang, Gregory J Bedwell, Satya P Singh, Hyun Jae Yu, Bjarki Arnarson, Parmit K Singh, Rajalingam Radhakrishnan, AidanDarian W Douglas, Zachary M Ingram, Christian Freniere, Onno Akkermans, Stefan G Sarafianos, Zandrea Ambrose, Yong Xiong, Praju V Anekal, Paula Montero Llopis, Vineet N KewalRamani, Ashwanth C Francis, Alan N Engelman, HIV-1 usurps mixed-charge domain-dependent CPSF6 phase separation for higher-order capsid binding, nuclear entry and viral DNA integration, Nucleic Acids Research, 2024;, gkae769, https://doi.org/10.1093/nar/gkae769 [Article]