Committed to meeting all of the flow cytometry needs for BIDMC and the external research community, the Flow Cytometry Core facility offers state-of-the-art instrumentation for routine flow cytometry and cell sorting for both sort and analysis capabilities of up to 18 fluorescent parameters.
The facility provides equipment, and consultation and data analysis services by dedicated personnel to both in-house and outside researchers. Areas of expertise include non-human primate (NHP) and infectious disease research.
The Flow Cytometry Core is committed to providing the latest software and lasers to better assist peer researchers. The Flow Cytometry Core facility is affiliated with Harvard University and is supported by the NIH Center for AIDS Research (CFAR).
- Data analysis and interpretation
- Experiment consultation and set-up
- Fluorescence activated cell sorting
- Panel development
- Antibody conjugation
- Assays (special development)
Keith Reeves, Ph.D.
Flow Core Lab Manager
Flow Core Lab Assistant
BD FACSAria II
COMS approved facility allows for sterile sorting of fixed and unfixed human, non-human primate, and other animal specimens for HCV and HIV/AIDS research. BD FACS Aria II cell sorter equipped within a Bioprotect IV LE biosafety cabinet operates with 4 solid state lasers: 405nm violet measuring up to 8 parameters, 488nm blue measuring up to 2 parameters in addition to FSC and SSC, 532nm green measuring up to 5 parameters, and a 628nm red measuring up to 3 parameters, allowing sorting panels of up to 18 fluorescent markers.
Two BD LSR II analyzers, each equipped with 4 solid state lasers: 405nm violet measuring up to 7 parameters, 488nm blue measuring up to 5 parameters in addition to FSC and SSC, a 640nm red measuring up to 3 parameters, and a 355 nm UV laser measuring 3 parameters allowing panels of up to 18 fluorescent markers.
BD LSRFortessa X14
Special research product with a choice of 3 solid state lasers a 405nm violet measuring up to 6 parameters, 488nm blue measuring up to 2 parameters in addition to FSC and SSC, a 561nm Yellow/Green measuring up to 4 parameters allowing panels of up to 12 fluorescent markers.
Siemens ADVIA 120 Hematology System
Multispecies analyzing system for complete blood count and differential counts
The HIV-1 Neutralizing Antibody Core provides in vitro HIV-1 neutralizing antibody assay services to BIDMC and the external research community. The core is GCLP-compliant; it serves as a resource for assessing the breadth and potency of HIV-1 neutralizing antibodies, and for characterizing the neutralization phenotype of HIV-1 isolates.
Investigators send monoclonal antibodies or serum samples from vaccinated or HIV-1-infected individuals to the core for in vitro testing using the validated TZM.bl neutralizing antibody assay. The core also has the capability to phenotype primary HIV-1 isolates for sensitivity to broadly neutralizing antibodies.
- In vitro testing of monoclonal antibodies or seurm samples
- Validated TZM.bl neutralizing antibody assay
- Phenotoyping of primary HIV-1 isolates
Michael Seaman, Ph.D.
The Harvard Catalyst Clinical Research Center (CRC) is a core facility provided to all BIDMC investigators as well as affiliated Harvard faculty for the conduct of clinical trials. The CRC provides contiguous space for inpatient, outpatient, and administrative support activities and is located on the 8th floors of the Gryzmish and Feldberg buildings at BIDMC.
The outpatient area consists of 12 rooms, including two general purpose rooms with beds and private bathrooms, one examination/minor procedure room, one interview room, and a room equipped for neurological testing, among other rooms. The inpatient unit consists of 8 beds in two double rooms and four single rooms. The unit is open for patient activity 24 hours per day, seven days per week.
CRC staff includes nurses, dieticians, a laboratory assistant, and a unit coordinator. Access to administrative space includes a conference room, investigator’s resource room, and use of the statistical programs on three computers located near the statistician’s office.
Learn more at https://catalyst.harvard.edu/