Development and validation of HIV SMRTcap for the characterization of HIV-1 reservoirs across tissues and subtypes

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Sadri G, Nadakal ST, Lauer W, Kos J, Singh PK, Elliott E, Kaiser CW, Ford EE, Richardson N, Shields KM, Hudson E, Linden NL, Danesh A, Powell J, Warburton P, Soto J, Emery M, Deikus G, Lee GQ, Lamers SL, Reynolds SJ, Galiwango R, Prodger JL, Tomusange S, Kityamuweesi T, Han T, Jones RB, Tobian AAR, Engelman AN, Sebra R, Morgello S, Redd AD, Sachs D, Rouchka E, Smith ML. Development and validation of HIV SMRTcap for the characterization of HIV-1 reservoirs across tissues and subtypes. PLoS Pathog. 2026 Jan 13;22(1):e1013171. doi: 10.1371/journal.ppat.1013171. PMID: 41529060; PMCID: PMC12851485.

HIV-1 nuclear import is selective and depends on both capsid elasticity and nuclear pore adaptability

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Hou Z, Shen Y, Fronik S, Shen J, Shi J, Xu J, Chen L, Hardenbrook N, Engelman AN, Aiken C, Zhang P. HIV-1 nuclear import is selective and depends on both capsid elasticity and nuclear pore adaptability. Nature Microbiology. 2025 Aug;10(8):1868-1885. doi: 10.1038/s41564-025-02054-z. Epub 2025 Jul 7.

Retrointegration2023—Papers from the 7th International Conference on Retroviral Integration

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Engelman AN, Grandgenett DP, Maertens GN, Yoder KE, Kvaratskhelia M. Retrointegration2023-Papers from the 7th International Conference on Retroviral Integration. Viruses. 2025 Jun 23;17(7):879. doi: 10.3390/v17070879.

Timed chromatin invasion during mitosis governs prototype foamy virus integration site selection and infectivity

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Lagadec F, Singh PK, Calmels C, Lapaillerie D, Lindemann D, Parissi V, Cherepanov P, Engelman AN, Lesbats P. Timed chromatin invasion during mitosis governs prototype foamy virus integration site selection and infectivity. Nucleic Acids Res. 2025 May 22;53(10):gkaf449. doi: 10.1093/nar/gkaf449.

Structural and mechanistic bases for resistance of the M66I capsid variant to lenacapavir

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Briganti, L., Annamalai, A. S., Bester, S. M., Wei, G., Andino-Moncada, J. R., Singh, S. P., Kleinpeter, A. B., Tripathi, M., Nguyen, B., Radhakrishnan, R., Singh, P. K., Greenwood, J., Schope, L. I., Haney, R., Huang, S.-W., Freed, E. O., Engelman, A. N., Francis, A. C., & Kvaratskhelia, M. (2025). Structural and mechanistic bases for resistance of the M66I capsid variant to lenacapavir. mBio, 16(5), e0361324. https://doi.org/10.1128/mbio.03613-24

CPSF6 promotes HIV-1 preintegration complex function

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Chaudhuri, E., Jang, S., Chakraborty, R., Radhakrishnan, R., Arnarson, B., Prakash, P., Cornish, D., Rohlfes, N., Singh, P. K., Shi, J., Aiken, C., Campbell, E., Hultquist, J., Balsubramaniam, M., Engelman, A. N., & Dash, C. (2025). CPSF6 promotes HIV-1 preintegration complex function. Journal of virology, 99(5), e0049025. https://doi.org/10.1128/jvi.00490-25

Spatiotemporal binding of cyclophilin A and CPSF6 to capsid regulates HIV-1 nuclear entry and integration

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Ingram, Z., Kline, C., Hughson, A. K., Singh, P. K., Fischer, H. L., Radhakrishnan, R., Sowd, G. A., Dos Santos, N. F. B., Ganser-Pornillos, B. K., Watkins, S. C., Kane, M., Engelman, A. N., & Ambrose, Z. (2025). Spatiotemporal binding of cyclophilin A and CPSF6 to capsid regulates HIV-1 nuclear entry and integration. mBio, 16(4), e0016925. https://doi.org/10.1128/mbio.00169-25

The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir

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Huang, S. W., Briganti, L., Annamalai, A. S., Greenwood, J., Shkriabai, N., Haney, R., Armstrong, M. L., Wempe, M. F., Singh, S. P., Francis, A. C., Engelman, A. N., & Kvaratskhelia, M. (2025). The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir. PLoS pathogens, 21(1), e1012862. https://doi.org/10.1371/journal.ppat.1012862

The nuclear localization signal of CPSF6 governs post-nuclear import steps of HIV-1 infection

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Rohlfes, N., Radhakrishnan, R., Singh, P. K., Bedwell, G. J., Engelman, A. N., Dharan, A., & Campbell, E. M. (2025). The nuclear localization signal of CPSF6 governs post-nuclear import steps of HIV-1 infection. PLoS pathogens, 21(1), e1012354. https://doi.org/10.1371/journal.ppat.1012354

HIV-1 usurps mixed-charge domain-dependent CPSF6 phase separation for higher-order capsid binding, nuclear entry and viral DNA integration

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Sooin Jang, Gregory J Bedwell, Satya P Singh, Hyun Jae Yu, Bjarki Arnarson, Parmit K Singh, Rajalingam Radhakrishnan, AidanDarian W Douglas, Zachary M Ingram, Christian Freniere, Onno Akkermans, Stefan G Sarafianos, Zandrea Ambrose, Yong Xiong, Praju V Anekal, Paula Montero Llopis, Vineet N KewalRamani, Ashwanth C Francis, Alan N Engelman, HIV-1 usurps mixed-charge domain-dependent CPSF6 phase separation for higher-order capsid binding, nuclear entry and viral DNA integration, Nucleic Acids Research, 2024;, gkae769, https://doi.org/10.1093/nar/gkae769