A peptide derived from lens epithelium-derived growth factor stimulates HIV-1 DNA integration and facilitates intasome structural studies

Li M, Chen X, Wang H, Jurado KA, Engelman AN, Craigie R. A peptide derived from lens epithelium-derived growth factor stimulates HIV-1 DNA integration and facilitates intasome structural studies. J Mol Biol 2020 Feb 13. pii: S0022-2836(20)30106-6. doi: 10.1016/j.jmb.2020.01.040. [Epub ahead of print]. PubMed PMID: 32061936.

Structural basis of second-generation inhibitor action and viral resistance

Cook NJ, Li W, Berta D, Badaoui D, Ballandras-Colas A, Nans A, Kotecha A, Rosta E, Engelman AN, Cherepanov P. Structural basis of second-generation inhibitor action and viral resistance. Science 2020 Feb 14;367(6479):806-10. doi: 10.1126/science.aay4919. Epub 2020 Jan 30. PubMed PMID: 32001525.

Multifaceted HIV integrase functionalities and therapeutic strategies for their inhibition.

Engelman, AN. Multifaceted HIV integrase functionalities and therapeutic strategies for their inhibition. J Biol Chem 2019 Oct 11;294(41):15137-57. doi: 10.1074/jbc.REV119.006901. Epub 2019 Aug 29. PubMed PMID: 31467082; PubMed Central PMCID: PMC6791320.

HIV-1 integrase tetramers are the antiviral target of pyridine-based allosteric integrase inhibitors.

Koneru PC, Francis AC, Deng N, Rebensburg SV, Hoyte AC, Lindenberger J, Adu-Ampratwum D, Larue RC, Wempe MF, Engelman AN, Lyumkis D, Fuchs JR, Levy RM, Melikyan GB, Kvaratskhelia M. HIV-1 integrase tetramers are the antiviral target of pyridine-based allosteric integrase inhibitors. Elife 2019 May 23;8. pii: e46344. doi: 10.7554/eLife.46344. PubMed PMID: 31120420; PubMed Central PMCID: PMC6581505.

Dominant negative MA-CA fusion protein is incorporated into HIV-1 cores and inhibits nuclear entry of viral preintegration complexes.

Anderson-Daniels J, Singh PK, Sowd GA, Li W, Engelman AN, Aiken C. Dominant negative MA-CA fusion protein is incorporated into HIV-1 cores and inhibits nuclear entry of viral preintegration complexes. J Virol 2019 Oct 15;93(21). pii: e01118-19. doi: 10.1128/JVI.01118-19. Print 2019 Nov 1. Epub 2019 Aug 14. PubMed PMID: 31413124; PubMed Central PMCID: _.

Disrupting MLV integrase:BET protein interaction biases integration into quiescent chromatin and delays but does not eliminate tumor activation in a MYC/Runx2 mouse model.

Loyola L, Achuthan V, Gilroy K, Borland G, Kilbey A, Mackay N, Bell M, Hay J, Aiyer S, Fingerman D, Villanueva RA, Cameron E, Kozak CA, Engelman AN, Neil J, Roth MJ. Disrupting MLV integrase:BET protein interaction biases integration into quiescent chromatin and delays but does not eliminate tumor activation in a MYC/Runx2 mouse model. PLoS Pathog 2019 Dec 9;15(12):e1008154. doi: 10.1371/journal.ppat.1008154. eCollection 2019 Dec. PubMed PMID: 31815961. PubMed Central PMCID: 6974304.

Differential role for phosphorylation in alternative polyadenylation function versus nuclear import of SR-like protein CPSF6.

Jang S, Cook NJ, Pye VE, Bedwell GJ, Dudek AM, Singh PK, Cherepanov P, Engelman AN. Differential role for phosphorylation in alternative polyadenylation function versus nuclear import of SR-like protein CPSF6. Nucleic Acids Res 2019 May 21;47(9):4663-4683. doi: 10.1093/nar/gkz206. Epub 2019 Mar 27. PubMed PMID: 30916345; PubMed Central PMCID: PMC6511849.

A HTRF based competitive binding assay for screening specific inhibitors of HIV-1 capsid assembly targeting the C-terminal domain of capsid.

Zhang DW, Luo RH, Xu L, Yang L-M, Xu X-S, Bedwell GJ, Engelman AN, Zheng Y-T, Chang S. A HTRF based competitive binding assay for screening specific inhibitors of HIV-1 capsid assembly targeting the C-terminal domain of capsid. Antiviral Res 2019 Sep;169:104544. doi: 10.1016/j.antiviral.2019.104544. Epub 2019 Jun 27. PubMed PMID: 31254557.

Cellular and molecular mechanisms of HIV-1 integration targeting.

Engelman AN, Singh PK. Cellular and molecular mechanisms of HIV-1 integration targeting. Cell Mol Life Sci 2018 July;75(14):2491-507. doi: 10.1007/s00018-018-2772-5. Epub 2018 Feb 7. PubMed PMID: 29417178; PubMed Central PMCID: PMC6004233.

Capsid-CPSF6 interaction licenses nuclear HIV-1 trafficking to sites of viral DNA integration.

Achuthan V, Perreira JM, Sowd GA, Puray-Chavez M, McDougall WM, Paulucci-Holthauzen A, Wu X, Fadel HJ, Poeschla EM, Multani AS, Hughes SH, Sarafianos SG, Brass AL, Engelman AN. Capsid-CPSF6 interaction licenses nuclear HIV-1 trafficking to sites of viral DNA integration. Cell Host Microbe 2018 Sep 12;24(3):392-404.e8. doi: 10.1016/j.chom.2018.08.002. Epub 2018 Aug 30. PubMed PMID: 30173955; PubMed Central PMCID: PMC6368089.