HIV-1 usurps mixed-charge domain-dependent CPSF6 phase separation for higher-order capsid binding, nuclear entry and viral DNA integration

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Sooin Jang, Gregory J Bedwell, Satya P Singh, Hyun Jae Yu, Bjarki Arnarson, Parmit K Singh, Rajalingam Radhakrishnan, AidanDarian W Douglas, Zachary M Ingram, Christian Freniere, Onno Akkermans, Stefan G Sarafianos, Zandrea Ambrose, Yong Xiong, Praju V Anekal, Paula Montero Llopis, Vineet N KewalRamani, Ashwanth C Francis, Alan N Engelman, HIV-1 usurps mixed-charge domain-dependent CPSF6 phase separation for higher-order capsid binding, nuclear entry and viral DNA integration, Nucleic Acids Research, 2024;, gkae769, https://doi.org/10.1093/nar/gkae769

HIV-1 Intasomes Assembled with Excess Integrase C-Terminal Domain Protein Facilitate Structural Studies by Cryo-EM and Reveal the Role of the Integrase C-Terminal Tail in HIV-1 Integration

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Li M, Li Z, Chen X, Cui Y, Engelman AN, Craigie R. HIV-1 Intasomes Assembled with Excess Integrase C-Terminal Domain Protein Facilitate Structural Studies by Cryo-EM and Reveal the Role of the Integrase C-Terminal Tail in HIV-1 Integration. Viruses. 2024; 16(7):1166. https://doi.org/10.3390/v16071166

Viral and host mediators of non-suppressible HIV-1 viremia

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Mohammadi A, Etemad B, Zhang X, Li Y, Bedwell GJ, Sharaf R, Kittilson A, Melberg M, Crain CR, Traunbauer AK, Wong C, Fajnzylber J, Worrall DP, Rosenthal A, Jordan H, Jilg N, Kaseke C, Giguel F, Lian X, Deo R, Gillespie E, Chishti R, Abrha S, Adams T, Siagian A, Dorazio D, Anderson PL, Deeks SG, Lederman MM, Yawetz S, Kuritzkes DR, Lichterfeld MD, Sieg S, Tsibris A, Carrington M, Brumme ZL, Castillo-Mancilla JR, Engelman AN, Gaiha GD, Li JZ. Viral and host mediators of non-suppressible HIV-1 viremia. Nat Med. 2023 Dec;29(12):3212-3223. doi: 10.1038/s41591-023-02611-1. Epub 2023 Nov 13. PMID: 37957382; PMCID: PMC10719098.

Pathways and Intersections: Multifaceted Approaches to Engage Individuals From Underrepresented and Marginalized Communities in HIV Research and Career Development

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Irie WC, Chitneni P, Glynn TR, Allen W, Chai PR, Engelman AN, Hurtado R, Li JZ, Li P, Lockman S, Marcus JL, Ogunshola FJ, Rönn MM, Haberer J, Ghebremichael M, Ciaranello A; Harvard University Center for AIDS Research Diversity, Equity, and Inclusion Working Group. Pathways and Intersections: Multifaceted Approaches to Engage Individuals From Underrepresented and Marginalized Communities in HIV Research and Career Development. J Acquir Immune Defic Syndr. 2023 Oct 1;94(2S):S116-S121. doi: 10.1097/QAI.0000000000003265. PMID: 37707858; PMCID: PMC10503030.

Capsid–host interactions for HIV-1 ingress

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Jang S, Engelman AN. Capsid-host interactions for HIV-1 ingress. Microbiol Mol Biol Rev. 2023 Dec 20;87(4):e0004822. doi: 10.1128/mmbr.00048-22. Epub 2023 Sep 26. PMID: 37750702; PMCID: PMC10732038.

The capsid lattice engages a bipartite NUP153 motif to mediate nuclear entry of HIV-1 cores

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Shen Q, Kumari S, Xu C, Jang S, Shi J, Burdick RC, Levintov L, Xiong Q, Wu C, Devarkar SC, Tian T, Tripler TN, Hu Y, Yuan S, Temple J, Feng Q, Lusk CP, Aiken C, Engelman AN, Perilla JR, Pathak VK, Lin C, Xiong Y. The capsid lattice engages a bipartite NUP153 motif to mediate nuclear entry of HIV-1 cores. Proc Natl Acad Sci U S A. 2023 Mar 28;120(13):e2202815120. doi: 10.1073/pnas.2202815120. Epub 2023 Mar 21. PMID: 36943880; PMCID: PMC10068764.

Modeling HIV-1 nuclear entry with nucleoporin-gated DNA-origami channels

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Shen Q, Feng Q, Wu C, Xiong Q, Tian T, Yuan S, Shi J, Bedwell GJ, Yang R, Aiken C, Engelman AN, Lusk CP, Lin C, Xiong Y. Modeling HIV-1 nuclear entry with nucleoporin-gated DNA-origami channels. Nat Struct Mol Biol. 2023 Apr;30(4):425-435. doi: 10.1038/s41594-023-00925-9. Epub 2023 Feb 20. PMID: 36807645; PMCID: PMC10121901.

The Drug-Induced Interface That Drives HIV-1 Integrase Hypermultimerization and Loss of Function

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Singer MR, Dinh T, Levintov L, Annamalai AS, Rey JS, Briganti L, Cook NJ, Pye VE, Taylor IA, Kim K, Engelman AN, Kim B, Perilla JR, Kvaratskhelia M, Cherepanov P. The Drug-Induced Interface That Drives HIV-1 Integrase Hypermultimerization and Loss of Function. mBio. 2023 Feb 28;14(1):e0356022. doi: 10.1128/mbio.03560-22. Epub 2023 Feb 6. PMID: 36744954; PMCID: PMC9973045.

Allosteric Integrase Inhibitor Influences on HIV-1 Integration and Roles of LEDGF/p75 and HDGFL2 Host Factors

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Singh PK, Li W, Bedwell GJ, Fadel HJ, Poeschla EM, Engelman AN. Allosteric Integrase Inhibitor Influences on HIV-1 Integration and Roles of LEDGF/p75 and HDGFL2 Host Factors. Viruses. 2022; 14(9):1883. https://doi.org/10.3390/v14091883

Spatial and Genomic Correlates of HIV-1 Integration Site Targeting

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Singh PK, Bedwell GJ, Engelman AN. Spatial and Genomic Correlates of HIV-1 Integration Site Targeting. Cells. 2022 Feb 14;11(4):655. doi: 10.3390/cells11040655. PMID: 35203306; PMCID: PMC8869898.