Differential role for phosphorylation in alternative polyadenylation function versus nuclear import of SR-like protein CPSF6.

Jang S, Cook NJ, Pye VE, Bedwell GJ, Dudek AM, Singh PK, Cherepanov P, Engelman AN. Differential role for phosphorylation in alternative polyadenylation function versus nuclear import of SR-like protein CPSF6. Nucleic Acids Res 2019 May 21;47(9):4663-4683. doi: 10.1093/nar/gkz206. Epub 2019 Mar 27. PubMed PMID: 30916345; PubMed Central PMCID: PMC6511849.

A HTRF based competitive binding assay for screening specific inhibitors of HIV-1 capsid assembly targeting the C-terminal domain of capsid.

Zhang DW, Luo RH, Xu L, Yang L-M, Xu X-S, Bedwell GJ, Engelman AN, Zheng Y-T, Chang S. A HTRF based competitive binding assay for screening specific inhibitors of HIV-1 capsid assembly targeting the C-terminal domain of capsid. Antiviral Res 2019 Sep;169:104544. doi: 10.1016/j.antiviral.2019.104544. Epub 2019 Jun 27. PubMed PMID: 31254557.

Cellular and molecular mechanisms of HIV-1 integration targeting.

Engelman AN, Singh PK. Cellular and molecular mechanisms of HIV-1 integration targeting. Cell Mol Life Sci 2018 July;75(14):2491-507. doi: 10.1007/s00018-018-2772-5. Epub 2018 Feb 7. PubMed PMID: 29417178; PubMed Central PMCID: PMC6004233.

Capsid-CPSF6 interaction licenses nuclear HIV-1 trafficking to sites of viral DNA integration.

Achuthan V, Perreira JM, Sowd GA, Puray-Chavez M, McDougall WM, Paulucci-Holthauzen A, Wu X, Fadel HJ, Poeschla EM, Multani AS, Hughes SH, Sarafianos SG, Brass AL, Engelman AN. Capsid-CPSF6 interaction licenses nuclear HIV-1 trafficking to sites of viral DNA integration. Cell Host Microbe 2018 Sep 12;24(3):392-404.e8. doi: 10.1016/j.chom.2018.08.002. Epub 2018 Aug 30. PubMed PMID: 30173955; PubMed Central PMCID: PMC6368089.

LEDGF/p75 is dispensable for hematopoiesis but essential for MLL-rearranged leukemogenesis.

El Ashkar S, Schwaller J, Pieters T, Goossens S, Demeulemeester J, Christ F, Van Belle S, Juege S, Boeckx N, Engelman A, Debyser Z, De Rijck J. LEDGF/p75 is dispensable for hematopoiesis but essential for MLL-rearranged leukemogenesis. Blood 2018 Jan 4;131(1):95-107. doi: 10.1182/blood-2017-05-786962. Epub 2017 Oct 30. PubMed PMID: 29084744; PubMed Central PMCID: PMC5755044.

Resistance to pyridine-based inhibitor KF116 reveals an unexpected role of integrase in HIV-1 Gag-Pol proteolytic processing.

Hoyte AC, Jamin AV, Koneru PC, Kobe MJ, Larue RC, Fuchs JR, Engelman AN, Kvaratskhelia M. Resistance to pyridine-based inhibitor KF116 reveals an unexpected role of integrase in HIV-1 Gag-Pol proteolytic processing. J Biol Chem 2017 Dec 1;292(48):19814-25. doi:10.1074/jbc.M117.816645. Epub 2017 Sep 28. PubMed PMID: 28972144; PubMed Central PMCID: PMC5712621.

Multiplex single-cell visualization of nucleic acids and protein during HIV infection.

Puray-Chavez M, Tedbury PR, Huber AD, Ukah OB, Yapo V, Liu D, Ji J, Wolf JJ, Engelman AN, Sarafianos SG. Multiplex single-cell visualization of nucleic acids and protein during HIV infection. Nat Commun 2017 Dec 1;8(1):1882. doi: 10.1038/s41467-017-01693-z. PubMed PMID: 29192235; PubMed Central PMCID: PMC5709414.

Molecular mechanisms for CFIm-mediated regulation of mRNA alternative polyadenylation.

Zhu Y, Wand X, Fouromand E, Jeong J, Qiao F, Sowd G, Engelman AN, Xie X, Hertel K, Shi Y. Molecular mechanisms for CFIm-mediated regulation of mRNA alternative polyadenylation. Mol Cell 2018 Jan 4;69(1):62-74.e4. doi: 10.1016/j.molcel.2017.11.031. Epub 2017 Dec 21. PubMed PMID: 29276085; PubMed Central PMCID: PMC5756121.

Inhibition of HIV-1 maturation via small molecule targeting of the amino-terminal domain in the viral capsid protein.

Wang W, Zhou J, Halambage UD, Jurado KA, Jamin AV, Wang Y, Engelman AN, Aiken C. Inhibition of HIV-1 maturation via small molecule targeting of the amino-terminal domain in the viral capsid protein. J Virol 2017 April 13;91(9). pii: e02155-16. doi: 10.1128/JVI.02155-16. Print 2017 May 1. PubMed PMID: 28202766; PubMed Central PMCID: PMC5391466.

Hematopoietic stem and progenitor cells from human pluripotent stem cells.

Sugimura R, Jha DK, Han A, Soria-Valles C, Lummertz da Rocha E, Lu Y-F, Goettel JA, Serrao E, Rowe RG, Malleshaiah M, Wong I, Sousa P, Zhu TN, Ditadi A, Keller G, Engelman AN, Snapper SB, Doulatov S, Daley GQ. Hematopoietic stem and progenitor cells from human pluripotent stem cells. Nature 2017 May 25;545(7655):432-8. doi: 10.1038/nature22370. Epub May 17. PubMed PMID: 28514439; PubMed Central PMCID: PMC5872146.