The competitive interplay between allosteric HIV-1 integrase inhibitor BI/D and LEDGF/p75 during the early stage of HIV-1 replication adversely affects inhibitor potency.

Feng L, Dharmarajan V, Serrao E, Hoyte A, Larue RC, Slaughter A, Sharma A, Plumb MR, Kessl JJ, Fuchs JR, Bushman FD, Engelman AN, Griffin PR, Kvaratskhelia M. The competitive interplay between allosteric HIV-1 integrase inhibitor BI/D and LEDGF/p75 during the early stage of HIV-1 replication adversely affects inhibitor potency. ACS Chem Biol 2016 Mar 20;11(5):1313-21. doi: 10.1021/acschembio.6b00167. Epub 2016 Mar 2. PubMed PMID: 26910179; PubMed Central PMCID: PMC4874862.

A critical role for alternative polyadenylation factor CPSF6 in targeting HIV-1 integration to transcriptionally active chromatin.

Sowd GA, Serrao E, Wang H, Wang W, Fadel HJ, Poeschla EM, Engelman AN. A critical role for alternative polyadenylation factor CPSF6 in targeting HIV-1 integration to transcriptionally active chromatin. Proc Natl Acad Sci U S A 2016 Feb 23;113(8):E1054-63. doi: 10.1073/pnas.1524213113. Epub 2016 Feb 8. PubMed PMID: 26858452; PubMed Central PMCID: PMC4776470.

Allosteric HIV-1 integrase inhibitors promote aberrant protein multimerization by directly mediating inter-subunit interactions: Structural and thermodynamic modeling studies.

Deng N, Hoyte A, Mansour YE, Mohamed MS, Fuchs JR, Engelman AN, Kvaratskhelia M, Levy R. Allosteric HIV-1 integrase inhibitors promote aberrant protein multimerization by directly mediating inter-subunit interactions: Structural and thermodynamic modeling studies. Protein Sci 2016 Nov;25(11):1911-1917. doi: 10.1002/pro.2997. Epub 2016 Aug 17. PubMed PMID: 27503276; PubMed Central PMCID: PMC5079246.

Amplification, next-generation sequencing, and genomic DNA mapping of retroviral integration sites.

Serrao E, Cherepanov P, Engelman AN. Amplification, next-generation sequencing, and genomic DNA mapping of retroviral integration sites. J Vis Exp 2016 Mar 22;109:e53840. doi: 10.3791/53840. PubMed PMID: 27023428; PubMed Central PMCID: PMC4829050.

Capsid-CPSF6 interaction is dispensable for HIV-1 replication in primary cells but is selected during virus passage in vivo.

Saito A, Henning MS, Serrao E, Dubose BN, Teng S, Huang J, Li X, Saito N, Roy SP, Siddiqui MA, Ahn J, Tsuji M, Hatziioannou T, Engelman AN, Yamashita M. Capsid-CPSF6 interaction is dispensable for HIV-1 replication in primary cells but is selected during virus passage in vivo. J Virol 2016 Jul 11;90(15):6918-35. doi: 10.1128/JVI.00019-16. Print 2016 Aug 1. PubMed PMID: 27307565; PubMed Central PMCID: PMC4944271.

Distribution and redistribution of HIV-1 nucleocapsid protein in immature, mature, and integrase-inhibited virions: A role for integrase in maturation.

Fontana J, Jurado KA, Cheng N, Ly NL, Fuchs JR, Gorelick RJ, Engelman AN, Steven AC. Distribution and redistribution of HIV-1 nucleocapsid protein in immature, mature, and integrase-inhibited virions: A role for integrase in maturation. J Virol 2015 Oct; 89(19):9765-80. doi: 10.1128/JVI.01522-15. Epub 2015 Jul 15. PubMed PMID: 26178982; PubMed Central PMCID: PMC4577894.

Embryonic lethality due to arrested cardiac development in Psip1/Hdgfrp2 double-deficient mice.

Wang H, Shun M-C, Dickson AK, Engelman AN. Embryonic lethality due to arrested cardiac development in Psip1/Hdgfrp2 double-deficient mice. PLoS One 2015; 10:e0137797. doi: 10.1371/journal.pone.0137797. eCollection 2015. PubMed PMID: 26367869; PubMed Central PMCID: PMC4569352.

Foreign DNA capture during CRISPR-Cas adaptive immunity.

Nuñez JK, Harrington LB, Kranzusch PJ, Engelman AN, Doudna JA. Foreign DNA capture during CRISPR-Cas adaptive immunity. Nature 2015 Nov 26; 527(7579):535-8. doi: 10.1038/nature15760. Epub 2015 Oct 21. PubMed PMID: 26503043; PubMed Central PMCID: PMC4662619.

Integrase-mediated spacer acquisition during CRISPR-Cas adaptive immunity.

Nuñez JK, Lee ASY, Engelman A, Doudna JA. Integrase-mediated spacer acquisition during CRISPR-Cas adaptive immunity. Nature 2015 Mar 12;519(7542):193-8. doi: 10.1038/nature14237. Epub 2015 Feb 18. PubMed PMID: 25707795; PubMed Central PMCID: PMC4359072.

Key determinants of target DNA recognition by retroviral intasomes.

Serrao E, Ballandras-Colas A, Cherepanov P, Maertens GN, Engelman AN. Key determinants of target DNA recognition by retroviral intasomes. Retrovirology 2015 Apr 30;12:39. doi: 10.1186/s12977-015-0167-3. PubMed PMID: 25924943; PubMed Central PMCID: PMC4422553.