Engineered murine HSCs reconstitute multi-lineage hematopoiesis and adaptive immunity.

Lu Y-F, Cahan P, Ross S, Sahalie J, Sousa PM, Hadland BK, Cai W, Serrao E, Engelman AN, Bernstein ID, Daley GQ. Engineered murine HSCs reconstitute multi-lineage hematopoiesis and adaptive immunity. Cell Rep 2016 Dec 20;17(12):3178-92. doi: 10.1016/j.celrep.2016.11.077. PubMed PMID: 28009288. PubMed Central PMCID: PMC5247798.

Establishment and reversal of HIV-1 latency in naïve and central memory CD4+ T cells in vitro.

Zerbato JM, Serrao E, Lenzi G, Kim B, Ambrose Z, Watkins SC, Engelman AN, Sluis-Cremer N. Establishment and reversal of HIV-1 latency in naïve and central memory CD4+ T cells in vitro. J Virol 2016 Aug 26;90(18):8059-73. doi: 10.1128/JVI.00553-16. Print 2016 Sep 15. PubMed PMID: 27356901; PubMed Central PMCID: PMC5008097.

Interactions of prototype foamy virus capsids with host polo-like kinases are important for efficient viral DNA integration.

Zurnic I, Hütter S, Rzeha U, Helbig R, Stanke N, Reh J, Müllers E, Hamann MV, Kern T, Gerresheim GK, Lindel F, Serrao E, Lesbats P, Engelman AN, Cherepanov P, Lindemann D. Interactions of prototype foamy virus capsids with host polo-like kinases are important for efficient viral DNA integration. PLoS Pathog 2016 Aug 31;12(8):e1005860. doi: 10.1371/journal.ppat.1005860. eCollection 2016 Aug. PubMed PMID: 27579920; PubMed Central PMCID: PMC5006980. Erratum in: PLoS Pathog. 2016 Oct 10;12(10 ):e1005956. doi: 10.1371/journal.ppat.1005956. PubMed PMID: 27723818; PubMed Central PMCID: PMC5056747.

Retroviral DNA integration.

Lesbats P, Engelman AN, Cherepanov P. Retroviral DNA integration. Chem Rev 2016;116(20):12730-57. doi: 10.1021/acs.chemrev.6B00125. Epub 2016 May 20. PubMed PMID: 27198982; PubMed Central PMCID: PMC5084067.

Roles of capsid-interacting host factors in multimodal inhibition of HIV-1 by PF74.

Saito A, Ferhadian D, Sowd GA, Serrao E, Shi J, Halambage UD, Teng S, Soto J, Siddiqui MA, Engelman AN, Aiken C, Yamashita M. Roles of capsid-interacting host factors in multimodal inhibition of HIV-1 by PF74. J Virol 2016 May 27;90(12):5808-23. doi: 10.1128/JVI.03116-15. Print 2016 Jun 15. PubMed PMID: 27076642; PubMed Central PMCID: PMC4886767.

The cleavage and polyadenylation specific factor 6 (CPSF6) subunit of the capsid-recruited pre-messenger RNA cleavage factor I (CFIm) complex mediates HIV-1 integration into genes.

Rasheedi S, Shun M-C, Serrao E, Sowd GA, Qian J, Hao C, Dasgupta T, Engelman AN, Skowronski J. The cleavage and polyadenylation specific factor 6 (CPSF6) subunit of the capsid-recruited pre-messenger RNA cleavage factor I (CFIm) complex mediates HIV-1 integration into genes. J Biol Chem 2016 May 27;291(22):11809-19. doi: 10.1074/jbc.M116.721647. Epub 2016 Mar 18. PubMed PMID: 26994143; PubMed Central PMCID: PMC4882448.

A new class of allosteric HIV-1 integrase inhibitors identified by crystallographic fragment screening of the catalytic core domain.

Patel D, Antwi J, Koneru PC, Serrao E, Forli S, Kessl JJ, Feng L, Deng N, Levy RM, Fuchs JR, Olson AJ, Engelman AN, Bauman JD, Kvaratskhelia M, Arnold E. A new class of allosteric HIV-1 integrase inhibitors identified by crystallographic fragment screening of the catalytic core domain. J Biol Chem 2016 Nov 4;291(45):23569-77. doi: 10.1074/jbc.M116.753384. Epub 2016 Sep 19. PubMed PMID: 27645997. PubMed Central PMCID: PMC5095411.

The competitive interplay between allosteric HIV-1 integrase inhibitor BI/D and LEDGF/p75 during the early stage of HIV-1 replication adversely affects inhibitor potency.

Feng L, Dharmarajan V, Serrao E, Hoyte A, Larue RC, Slaughter A, Sharma A, Plumb MR, Kessl JJ, Fuchs JR, Bushman FD, Engelman AN, Griffin PR, Kvaratskhelia M. The competitive interplay between allosteric HIV-1 integrase inhibitor BI/D and LEDGF/p75 during the early stage of HIV-1 replication adversely affects inhibitor potency. ACS Chem Biol 2016 Mar 20;11(5):1313-21. doi: 10.1021/acschembio.6b00167. Epub 2016 Mar 2. PubMed PMID: 26910179; PubMed Central PMCID: PMC4874862.

A critical role for alternative polyadenylation factor CPSF6 in targeting HIV-1 integration to transcriptionally active chromatin.

Sowd GA, Serrao E, Wang H, Wang W, Fadel HJ, Poeschla EM, Engelman AN. A critical role for alternative polyadenylation factor CPSF6 in targeting HIV-1 integration to transcriptionally active chromatin. Proc Natl Acad Sci U S A 2016 Feb 23;113(8):E1054-63. doi: 10.1073/pnas.1524213113. Epub 2016 Feb 8. PubMed PMID: 26858452; PubMed Central PMCID: PMC4776470.

Allosteric HIV-1 integrase inhibitors promote aberrant protein multimerization by directly mediating inter-subunit interactions: Structural and thermodynamic modeling studies.

Deng N, Hoyte A, Mansour YE, Mohamed MS, Fuchs JR, Engelman AN, Kvaratskhelia M, Levy R. Allosteric HIV-1 integrase inhibitors promote aberrant protein multimerization by directly mediating inter-subunit interactions: Structural and thermodynamic modeling studies. Protein Sci 2016 Nov;25(11):1911-1917. doi: 10.1002/pro.2997. Epub 2016 Aug 17. PubMed PMID: 27503276; PubMed Central PMCID: PMC5079246.